Cellobiose Uptake in the Hyperthermophilic Archaeon Pyrococcus furiosus Is Mediated by an Inducible, High-Affinity ABC Transporter

AUTOR(ES)

Koning, Sonja M.

FONTE

American Society for Microbiology

RESUMO

The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds cellobiose but also cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, and sophorose. The cbtA gene was cloned and functionally expressed in Escherichia coli. cbtA belongs to a gene cluster that encodes a transporter that belongs to the Opp family of ABC transporters.

Documentos Relacionados

• Caspofungin Uptake Is Mediated by a High-Affinity Transporter in Candida albicans
• The Adhesion-Associated sca Operon in Streptococcus gordonii Encodes an Inducible High-Affinity ABC Transporter for Mn2+ Uptake
• High-affinity maltose/trehalose transport system in the hyperthermophilic archaeon Thermococcus litoralis.
• Heat Shock Response by the Hyperthermophilic Archaeon Pyrococcus furiosus
• Characterization of an Aminoacylase from the Hyperthermophilic Archaeon Pyrococcus furiosus