Cellular fatty acids in Fusobacterium species as a tool for identification.
AUTOR(ES)
Tunér, K
RESUMO
Identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests because of the relative biochemical inactivity of these bacteria. Even the finding of pointed, thin gram-negative cells on Gram-stained slides can no longer be relied on for identification of Fusobacterium nucleatum, since at least four other species of fusobacteria have been seen to exhibit similar morphology. We examined 46 clinical isolates and six American Type Culture Collection type strains of fusobacteria by conventional methods and by the Microbial ID Systems MIDI software package for analyzing cellular fatty acid patterns measured by capillary column gas-liquid chromatography. Distinctive patterns of major fatty acids could be used to reliably identify most clinical isolates to the species level. The MIDI system identified 89% of the isolates correctly and provides an alternative to conventional methods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=270637Documentos Relacionados
- Suicide phenomenon in mesophilic aeromonads as a basis for species identification.
- Identification of Achromobacter species by cellular fatty acids and by production of keto acids.
- Cellular fatty acids of Capnocytophaga species.
- Gas-liquid chromatography of cellular fatty acids for identification of staphylococci.
- Application of gas-liquid chromatographic analysis of cellular fatty acids for species identification and typing of coagulase-negative staphylococci.
