Cellular location of alpha-hemolysin in Staphylococcus aureus.
AUTOR(ES)
Nugent, K M
RESUMO
Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415280Documentos Relacionados
- Expression of a cloned Staphylococcus aureus alpha-hemolysin determinant in Bacillus subtilis and Staphylococcus aureus.
- Chromosomal map location of the alpha-hemolysin structural gene in Staphylococcus aureus NCTC 8325.
- PURIFICATION OF STAPHYLOCOCCAL ALPHA-HEMOLYSIN
- Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12.
- MECHANISM OF ACTION OF STAPHYLOCOCCAL ALPHA-HEMOLYSIN I. : Some Factors Influencing the Measurement of Alpha-Hemolysin