Cellular sequences are present in the presumptive avian myeloblastosis virus genome.
AUTOR(ES)
Souza, L M
RESUMO
EcoRI restriction endonuclease fragments from a lambda proviral DNA hybrid containing the entire presumptive avian myeloblastosis virus (AMV) provirus, and from a lambda proviral hybrid containing a partial myeloblastosis-associated virus type 1 (MAV-1)-like provirus were compared by heteroduplex analysis. The cloned presumptive AMV provirus was also analyzed by electron microscopy, using R-loop formation with purified 35S RNA isolated from virions of the standard AMV complex. The results indicate that the putative AMV genome contains a segment absent in its MAV-1-like helper virus. This segment represents a substitution in the region of the genome that in MAV-1 virus is occupied by the envelope gene and is approximately 900 +/- 160 nucleotide pairs in length. Hybridization of specific probes from the presumptive AMV genome to Southern blots of EcoRI-digested cellular DNA has revealed that these substituted sequences are homologous to chicken and duck DNA that is not related to chicken endogenous proviral sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350020Documentos Relacionados
- Terminally repeated sequences in the avian sarcoma virus RNA genome.
- DNA of avian myeloblastosis-associated virus type 2 integrates at multiple sites in the chicken genome.
- DNA sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome.
- Identification of the avian myeloblastosis virus genome. I. Identification of restriction endonuclease fragments associated with acute myeloblastic leukemia.
- Characterization of cellular sequences flanking an integrated bovine leukemia virus genome.
