Celulas formadoras de colonias (CFCs) e produção de fatores estimuladores de colonias (CSFs), apos infecção, em animais expostos ao chumbo

AUTOR(ES)

Claudia Bincoletto

DATA DE PUBLICAÇÃO

1993

RESUMO

In this work we have investigated the effects of lead exposure on the growth and differentiation of hematopoietic cells from bone marrow, the so called colony forming cells (CFCs), in normal and infected mice. We also studied the effects of this exposure the serum activity of hemopoietic colony stimulating factors (CSFs), as well as, the survival of these mice after the infection. For this purpose, we used the technique for the clonal culture of hemopoietic cells in semi-solid medium. Mice were infected with the bacteria Listeria monocytogenes after treatment with lead acetate. Two strains of mice were used: Balbcj (susceptible to Listeria monocytogenes) and C57BI10 (resistent to this bacteria). The doses of lead acetate were: 1300, 130 and 13ppm in periods of 70, 30 and 10 days. At end of this treatment, mice were infected and killed 24, 48 and 72 hours after the inoculation of the bacteria. The survival of these mice was determineted after a period of ten days. The suppressives effects of lead were observed in both strains in the three different periods studied. The dose-response relationship was observed with the 3 doses of lead used in relation to the effects of the infection, however, we observed that in the resistant strain the suppressives effects were overcome 48 hours after the administration of the baçteria. In the susceptible strain the suppressives effects of the infection were evident in the 3 periods studied. No changes were observed in the serum activity of CSFs due to the administration of lead, thus suggesting that this metal acts by a direct action on the myelopoietic cells. A significant decrease in host resistence, as measured by the mortality rate, was found when both strain of mice, after treatment with 1300ppm of lead for 30 days, were challenged with sub-lethal doses of Listeria monocytogenes.

ASSUNTO(S)

listeria monocytogenes intoxicação por chumbo camundongos celulas hematopoieticas primitivas

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