Characterisation of Liver membrane autoantibodies determined by indirect immunofluorescence.
AUTOR(ES)
Tage-Jensen, U
RESUMO
Indirect immunofluorescence studies were performed using sera and IgG-Fab2 fragments from patients with chronic active hepatitis (CAH) who were positive for a liver membrane antibody (LMA). The specificity was investigated using hepatocytes from humans as well as rabbit, rat, guinea pig and monkey. Only sera also positive for smooth muscle antibody gave staining of lymphocytes and absorption with F-actin from rabbit muscle abolished this as well as all other smooth muscle staining without influencing LMA. It was concluded that LMA, routinely detected by indirect immunofluorescence using rabbit hepatocytes, represents specific binding to non-species-specific membrane antigens which are normal constituents of human hepatocytes. The antigen is separately located, and not cross-reactive with F-actin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=497537Documentos Relacionados
- Classification of smooth muscle autoantibodies detected by immunofluorescence.
- The detection of canine autoantibodies to thyroid antigens by enzyme-linked immunosorbent assay, hemagglutination and indirect immunofluorescence.
- Visualization of microtubules of cells in situ by indirect immunofluorescence.
- Identification of kappa opioid receptors in the immune system by indirect immunofluorescence.
- Antagonism among the normal anaerobic bacteria of the mouse gastrointestinal tract determined by immunofluorescence.