Characterisation of non-histone nuclear proteins cross reactive with purified rheumatoid factors.
AUTOR(ES)
Mason, J C
RESUMO
In order to examine the interactions between isolated rheumatoid factors (RFs) and cell nuclear antigens we have prepared 10 RFs by affinity chromatography against IgG coupled to Sepharose. Of these, seven cross reacted with cell nuclei on indirect immunofluorescence. The nuclear antigen appeared to be DNA histones by indirect immunofluorescence on rat liver, though on immunoblotting the rheumatoid factors also reacted with three non-histone polypeptides which were identified in the soluble fraction of nuclear extracts. We were unable to show any relationship between these polypeptides and rheumatoid arthritis nuclear antigen. These reactions represent a hitherto unrecognised phenomenon, which extends the range of antigens recognised by rheumatoid factors. We suggest that the immunopathogenic significance of RFs may not be restricted to their reactivity with IgG, and that non-histone nuclear proteins merit further investigation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1001633Documentos Relacionados
- The premelting of nucleoprotein: role of non-histone proteins
- Effect of non-histone proteins on thermal transition of chromatin and of DNA.
- Studies on the association of the high mobility group non-histone chromatin proteins with isolated nucleosomes.
- Selective effects of inhibitors of protein synthesis on metabolism of nuclear anc cytoplasmic proteins: evidence for coordinate synthesis of non-histone chromosomal proteins.
- Chicken non-histone chromosomal protein HMG-17 cDNA sequence.