Characteristics of Teratomas Regenerated in Vitro from Octopine-Type Crown Gall

AUTOR(ES)

Owens, Lowell D.

RESUMO

Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.

Documentos Relacionados

• Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines.
• Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
• Competition of Octopine-Catabolizing Pseudomonas spp. and Octopine-Type Agrobacterium tumefaciens for Octopine in Chemostats
• Relationship between the limited and wide host range octopine-type Ti plasmids of Agrobacterium tumefaciens.
• Wound-Released Chemical Signals May Elicit Multiple Responses from an Agrobacterium tumefaciens Strain Containing an Octopine-Type Ti Plasmid