Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments.
AUTOR(ES)
Hatfield, D L
RESUMO
A naturally occurring opal suppressor serine tRNA has been purified from chicken liver and used as a probe to isolate the corresponding gene from a library of chicken DNA in bacteriophage lambda. This minor tRNA is encoded by a single-copy gene that is not part of a tRNA gene cluster. DNA sequence analysis of the gene and its flanking DNA segments shows that the gene is encoded in an 87-base-pair segment without intervening sequences and specifies a tRNA that reads the termination codon UGA. This gene has additional nucleotides in the 5' internal promoter region but has a normal 3' internal promoter sequence and the usual termination signal.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=384163Documentos Relacionados
- Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene.
- Conserved sequences in both coding and 5' flanking regions of mammalian opal suppressor tRNA genes.
- Unique pathway of expression of an opal suppressor phosphoserine tRNA.
- Isolation and nucleotide sequence of a plant tRNA gene: petunia asparagine tRNA.
- Nucleotide sequence of a second glutamine tRNA gene in wheat mitochondrial DNA.
