Characterization of 2,3-dihydroxybenzoic acid from Nocardia asteroides GUH-2.
AUTOR(ES)
Feistner, G J
RESUMO
Culture filtrates of virulent Nocardia asteroides GUH-2 after growth in acetate minimal medium displayed an absorbance maximum at 320 nm. After isolation by polyamide extraction and anion chromatography, a UV-active compound with this absorbance was shown to be 2,3-dihydroxybenzoic acid (DHB) by nuclear magnetic resonance, gas chromatographic, and mass spectrometric techniques. DHB production under several culture conditions was quantified by a standard high-pressure liquid chromatography assay. Under iron deficiency conditions, N. asteroides GUH-2 excreted up to 11 mg of DHB per liter into the culture medium. No DHB was detected when N. asteroides GUH-2 was grown in an iron-rich medium. With the less virulent strain N. asteroides 10905, DHB was not found under any condition tested.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213697Documentos Relacionados
- Effect of growth stage on mycolic acid structure in cell walls of Nocardia asteroides GUH-2.
- Relationship among cell wall composition, stage of growth, and virulence of Nocardia asteroides GUH-2.
- Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore.
- Enzymatic determination of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid.
- 2,3-Dihydroxybenzoic acid, a new growth factor for multiple aromatic auxotrophs.
