Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1.
AUTOR(ES)
Poon, A P
RESUMO
The UL15 gene of herpes simplex virus 1 consists of two exons and is highly conserved among the herpesviruses sequenced to date. Other than its homology to a phage protein involved in the packaging of DNA, nothing is known of its function. This report concerns the isolation of a temperature-sensitive mutant with a mutation mapping in the UL15 open reading frame. Cells infected with the parent, mutant, and rescued viruses all make DNA at the nonpermissive temperature. Direct analyses of the DNA and electron microscopic studies indicate that although viral DNA is made, it is not packaged into capsids present in nuclei. These studies suggest that UL15 may be involved in the packaging of viral DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=237833Documentos Relacionados
- Isolation and preliminary characterization of a phosphonoacetic acid-resistant and temperature-sensitive mutant of herpes simplex virus type 1.
- Biochemical transformation by temperature-sensitive mutants of herpes simplex virus type 1.
- Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
- Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.
- Deletion of the VP16 open reading frame of herpes simplex virus type 1.