Characterization of Circular Plasmid Dimers in Borrelia burgdorferi
AUTOR(ES)
Tilly, Kit
FONTE
American Society for Microbiology
RESUMO
We have inactivated the ospC, oppAIV, and guaB genes on the 26-kb circular plasmid of Borrelia burgdorferi (cp26) by allelic exchange. On several occasions following such transformations, the cp26 of transformants had an aberrant mobility through agarose gels. Characterization of these cp26 molecules showed that the plasmid had dimerized. These dimers were quite stable during either selective or nonselective passage. Subsequent transformations with dimer DNA supported the hypothesis that in B. burgdorferi, transforming cp26 DNA most likely does not displace the resident homologous plasmid but rather must recombine in order to donate sequences that it carries. These serendipitous findings provide a mechanism for obtaining heterozygous complemented control strains when mutant phenotypes are characterized.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107627Documentos Relacionados
- Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease.
- Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.
- Linear and Circular Plasmid Content in Borrelia burgdorferi Clinical Isolates
- Conservation of Plasmid Maintenance Functions between Linear and Circular Plasmids in Borrelia burgdorferi
- Directed insertion of a selectable marker into a circular plasmid of Borrelia burgdorferi.
