Characterization of lipopolysaccharide fractions and their interactions with cells and model membranes.

AUTOR(ES)
RESUMO

The role of the length of the O-antigen polysaccharide side chain of bacterial lipopolysaccharide (LPS) in biological and model membrane systems was investigated. LPS from Salmonella typhimurium ATCC 14028 was chromatographed on a Sephadex G-200 column in the presence of sodium deoxycholate and separated into three fractions on the basis of molecular size. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot (immunoblot), and chemical analyses indicated that these fractions differed from each other primarily in the number of repeating units in the O-antigen polysaccharide side chain. In a biological system fractions 2 and 3 had the same effects to induce mitogenesis in murine lymphocytes, but fraction 1 was less effective than the other two fractions. In a model membrane system, LPS induced changes in small unilamellar vesicles (SUVs) which were measured by changes in the behavior of a fluorescent probe, 1,6-diphenylhexa-1,3,5-triene (DPH), and interaction of increasing amounts of all LPS fractions with SUVs gradually increased DPH anisotropy. Fractions 2 and 3 had similar effects on the SUVs as detected by changes in DPH anisotropy, while fraction 1 had almost twice as much activity as the other two fractions. These results suggest that the polysaccharide side chain of LPS may modulate the ability of biologically active lipid A to interact with cells and model membranes. In addition, factors other than changes in membrane fluidity may play a role in mediating LPS-induced cell activation.

ACESSO AO ARTIGO

Documentos Relacionados