Characterization of the Role of the ToxR-Modulated Outer Membrane Porins OmpU and OmpT in Vibrio cholerae Virulence
AUTOR(ES)
Provenzano, Daniele
FONTE
American Society for Microbiology
RESUMO
ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viability and virulence factor expression, in-frame chromosomal deletions were constructed in the coding sequences of ompU and ompT of V. cholerae. Two separate deletions were introduced into ompU; the first (small) deletion, ΔompU1, removed the coding sequence for 84 internal amino acids (aa), while the second (large) deletion, ΔompU2, removed the coding sequence for the entire amino-terminal 274 aa. The ΔompU1 strain had a growth defect that could not be complemented by episomal expression of full-length ompU. In contrast, a strain with ΔompU2 displayed wild-type growth kinetics in rich media, suggesting that this is the true phenotype of a strain lacking OmpU and that the truncated OmpU protein, rather than the absence of OmpU, may be the cause for the ΔompU1 phenotype. A large deletion removing the coding sequence for the entire N-terminal 273 aa of OmpT (ΔompT) was also constructed in wild-type as well as ΔtoxR and ΔompU2 strains, and these strains displayed wild-type growth kinetics in rich media. However, the ΔompU2 strain was deficient for growth in deoxycholate compared to wild-type, ΔompT, and ΔompU2 ΔompT strains, reinforcing a positive role for the OmpU porin and a negative role for the OmpT porin in V. cholerae resistance to anionic detergents. The ΔompU2, ΔompT, and ΔompU2 ΔompT strains exhibited wild-type levels of in vitro virulence factor expression and resistance to polymyxin B and serum and in vivo colonization levels similar to a wild-type strain in the infant mouse intestine. Our results demonstrate that (i) OmpU and OmpT are not essential proteins, as was previously thought; (ii) these porins contribute to V. cholerae resistance to anionic detergents; and (iii) OmpU and OmpT are not essential for virulence factor expression in vitro or intestinal colonization in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95243Documentos Relacionados
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