Characterization of two SV40 early mRNAs and evidence for a nuclear "prespliced" RNA species.

AUTOR(ES)

May, E

RESUMO

Using in vitro translation of sucrose-gradient fractionated cytoplasmic mRNA from SV40-infected cells, we have shown that a deletion in the region mapping between 0.54--0.59 reduced the size of mRNA for small-t but not the size of mRNA for large-T. Mutants with a deletion in this region were shown to produce in vivo either shortened small-t or no small-t, and normal large-T. Similarly, in vitro translation of poly(A)+cytoplasmic RNA from cells infected with these mutants gave the same results. On the other hand in vitro translation of poly(A)+nuclear RNA from the mutants which made no small-t produced a small-t derivative possibly synthesized from a prespliced RNA species. We have also shown that poly(A)+nuclear RNA from mutant dl 2122 produced two small-t related proteins: one of these (MW: 11K) probably represents the product of a "prespliced" RNA, the other (MW: 17K) which is also found in the cytoplasm represents the product of the mutant specific small-t mRNA.

Documentos Relacionados

• Characterization of two SV40 early mRNAs and evidence for a nuclear “prespliced” RNA species
• Alternative splicing of SV40 early pre-mRNA in vitro.
• Spliced early mRNAs of simian virus 40
• Independent expression of the transforming amino-terminal domain of SV40 large I antigen from an alternatively spliced third SV40 early mRNA.
• Electrophoretic analysis of covalently closed SV40 DNA: Boltzmann distributions of DNA species.