CHEMICAL STUDIES ON CELLULAR COMPONENTS OF BORDETELLA PERTUSSIS III. : Isolation of Highly Potent Toxin from Bordetella Pertussis
AUTOR(ES)
Onoue, Kaoru
RESUMO
Onoue, Kaoru (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. III. Isolation of highly potent toxin from Bordetella pertussis. J. Bacteriol. 86:648–655. 1963.—The thermolabile toxin of Bordetella pertussis was extracted at alkaline pH with 0.15 m saline from the disrupted cells. The toxin was purified successively by calcium phosphate gel treatment, ammonium sulfate fractionation, precipitation with potassium phosphate at alkaline pH, and finally by chromatography on a diethylaminoethyl cellulose column. Although the purified toxin still contained a small amount of agglutinin-absorbing activity, and inhomogeneity was detected by the agar gel diffusion test, the lethal and skin-necrotizing activities were much higher than those previously reported. The data obtained suggest that the toxin is proteinaceous.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=278497Documentos Relacionados
- CHEMICAL STUDIES ON CELLULAR COMPONENTS OF BORDETELLA PERTUSSIS I. : Purification and Properties of Agglutinogen
- STUDIES ON TOXIN PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE E III. : Characterization of Toxin Precursor
- Leukocytosis-Promoting Factor of Bordetella pertussis III. Its Identity with Protective Antigen
- Cellular pertussis vaccine containing a Bordetella pertussis strain that produces a nontoxic pertussis toxin molecule.
- Antibacterial Agents and Release of Periplasmic Pertussis Toxin from Bordetella pertussis
