Chemostat Culture of Escherichia coli K-12 Limited by the Activity of Alkaline Phosphatase
AUTOR(ES)
King, Stagg L.
RESUMO
The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of β-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is discussed in terms of the so-called Flip-Flop mechanism.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=187165Documentos Relacionados
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