Chick-Erythrocyte Nucleus Reactivation in Heterokaryons: Suppression by Inhibitors of Proteolytic Enzymes

AUTOR(ES)

Darzynkiewicz, Zbigniew

RESUMO

Reactivation of chick-erythrocyte nuclei in heterokaryons (obtained by Sendai virus-induced fusion of chick erythrocytes with HeLa cells) is suppressed by specific inhibitors of trypsin and trypsin-like enzymes. N-α-tosyl-L-lysyl-chloromethane and N-α-tosyl-L-arginine methylester inhibit erythrocyte nuclear enlargement and suppress RNA and DNA synthesis in nuclei of erythrocytes and HeLa cells in heterokaryons at concentrations that only minimally influence individual HeLa cells or HeLa homokaryons.

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