Chloroplast endoribonuclease p54 involved in RNA 3'-end processing is regulated by phosphorylation and redox state.

AUTOR(ES)

Liere, K

RESUMO

Chloroplast RNA-binding protein p54 is an endoribonuclease required for 3'end-processing of plastid precursor transcripts. We find that purified p54 can serve as a phosphate acceptor for protein kinases in vitro. Both the processing and RNA-binding activities of p54 are enhanced by phosphorylation and decreased by dephosphorylation. In addition, the enzyme is activated by the oxidized form of glutathione and inhibited by the reduced form, whereas other redox reagents that were tested showed no effect. Kinase treatment of p54 prior to oxidation by glutathione resulted in highest levels of activation, suggesting that phosphorylation and redox state act together to control p54 activity in vitro and possibly also in vivo.

Documentos Relacionados

• Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.
• Regulated adenovirus mRNA 3'-end formation in a coupled in vitro transcription-processing system.
• Alternative 3′-end processing of U5 snRNA by RNase III
• Transcription Termination and 3′-End Processing of the Spliced Leader RNA in Kinetoplastids
• Transcription termination and RNA processing in the 3'-end spacer of mouse ribosomal RNA genes.