Chloroplast Grana Membrane Carboxyl Groups: Their Involvement in Membrane Association 1

AUTOR(ES)

Berg, S.

RESUMO

Chloroplast membrane carboxyl groups were modified by carbodiimide activation followed by glycine methyl ester substitution, leaving the derivatized group uncharged. This charge alteration induced a number of effects similar to addition of salts to control chloroplasts suspended in a low salt medium. These include: (a) restacking or multiple membrane association in low salt-treated chloroplasts that lack grana stacks, (b) protection against polycation inhibition of photosystem I electron transfer, (c) reduction of the amount of polycations bound to the membranes, and (d) increased 90° light scattering due to membrane conformational changes. Carboxyl modification also altered acid-induced conformational changes.

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