Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.
AUTOR(ES)
Hayes, R
RESUMO
In the absence of efficient transcription termination correct 3'-end processing is an essential step in the synthesis of stable chloroplast mRNAs in higher plants. We show here that 3'-end processing in vitro involves endonucleolytic cleavage downstream from the mature terminus, followed by exonucleolytic processing to a stem-loop within the 3'-untranslated region. These processing steps require a high molecular weight complex that contains both endoribonucleases and an exoribonuclease. In the presence of ancillary RNA binding proteins the complex correctly processes the 3'-end of precursor RNA. In the absence of these ancillary proteins 3'-end maturation is prevented and plastid mRNAs are degraded. Based on these results we propose a novel mechanism for the regulation of mRNA 3'-end processing and stability in chloroplasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=450011Documentos Relacionados
- Chloroplast mRNA 3' end processing requires a nuclear-encoded RNA-binding protein.
- Regulated adenovirus mRNA 3'-end formation in a coupled in vitro transcription-processing system.
- Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
- Chloroplast endoribonuclease p54 involved in RNA 3'-end processing is regulated by phosphorylation and redox state.
- REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation.