CHOP-Dependent Stress-Inducible Expression of a Novel Form of Carbonic Anhydrase VI
AUTOR(ES)
Sok, John
FONTE
American Society for Microbiology
RESUMO
CHOP (also called GADD153) is a stress-inducible nuclear protein that dimerizes with members of the C/EBP family of transcription factors and was initially identified as an inhibitor of C/EBP binding to classic C/EBP target genes. Subsequent experiments suggested a role for CHOP-C/EBP heterodimers in positively regulating gene expression; however, direct evidence that this is the case has so far not been uncovered. Here we describe the identification of a positively regulated direct CHOP-C/EBP target gene, that encoding murine carbonic anhydrase VI (CA-VI). The stress-inducible form of the gene is expressed from an internal promoter and encodes a novel intracellular form of what is normally a secreted protein. Stress-induced expression of CA-VI is both CHOP and C/EBPβ dependent in that it does not occur in cells deficient in either gene. A CHOP-responsive element was mapped to the inducible CA-VI promoter, and in vitro footprinting revealed binding of CHOP-C/EBP heterodimers to that site. Rescue of CA-VI expression in c/ebpβ−/− cells by exogenous C/EBPβ and a shorter, normally inhibitory isoform of the protein known as LIP suggests that the role of the C/EBP partner is limited to targeting the CHOP-containing heterodimer to the response element and points to a preeminent role for CHOP in CA-VI induction during stress.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=83907Documentos Relacionados
- Stress-Inducible Cellular Responses
- TRB3, a novel ER stress-inducible gene, is induced via ATF4–CHOP pathway and is involved in cell death
- Identification of a novel p53 promoter element involved in genotoxic stress-inducible p53 gene expression.
- Molecular Characterization of a Stress-Inducible Gene from Lactobacillus helveticus
- Evaluation of stress-inducible hsp90 gene expression as a potential molecular biomarker in Xenopus laevis