Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cerevisiae.
AUTOR(ES)
Sledziewski, A
RESUMO
In the present study we have analyzed the kinetics of DNase I digestion of the two alcohol dehydrogenase (ADH) genes within yeast nuclei. We have found that in yeast grown on glucose the constitutively transcribed ADCI gene is much more sensitive to DNase I digestion than is the repressible ADR2 gene. In yeast grown on ethanol, both genes are transcribed and both exhibit the same sensitivity to DNase I attack. We have also found and mapped DNase I hypersensitive sites near the 5' ends of constitutive and repressible ADH genes. These sites are well correlated with the position at which transcription is initiated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345704Documentos Relacionados
- Comparison of the levels of the 21S mitochondrial rRNA in derepressed and glucose-repressed Saccharomyces cerevisiae.
- Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate.
- Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae.
- Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.
- Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae.