Chromatofocusing in the purification of staphylococcal enterotoxin D.
AUTOR(ES)
Lei, Z
RESUMO
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266573Documentos Relacionados
- Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.
- Increased susceptibility to staphylococcal enterotoxin B intoxication in mice primed with actinomycin D.
- Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.
- Identification of a Fourth Staphylococcal Enterotoxin, Enterotoxin D
- PURIFICATION OF TYPE B STAPHYLOCOCCAL ENTEROTOXIN1
