Chromosomal context dependence of a eukaryotic recombinational hot spot.
AUTOR(ES)
Ponticelli, A S
RESUMO
The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48209Documentos Relacionados
- Rec-Mediated Recombinational Hot Spot Activity in Bacteriophage Lambda II. a Mutation Which Causes Hot Spot Activity
- Strong structural effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.
- The replication termination signal terB of the Escherichia coli chromosome is a deletion hot spot.
- Rec-Mediated Recombinational Hot Spot Activity in Bacteriophage Lambda. I. Hot Spot Activity Associated with Spi- Deletions and bio Substitutions
- Single adduct mutagenesis: strong effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.
