Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli

AUTOR(ES)

Cozzarelli, N. R.

RESUMO

Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763–1766. 1966.—A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1–methionine-1–glycerol kinase–isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.

Documentos Relacionados

• Chromosomal Location of a Gene for Fructose 6-Phosphate Kinase in Escherichia coli
• Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.
• Chromosomal location of the gene encoding phosphoribosylpyrophosphate synthetase in Escherichia coli.
• Glycerol Kinase, the Pacemaker for the Dissimilation of Glycerol in Escherichia coli1
• Location of the Structural Gene for Fructose-1,6-Diphosphate Aldolase in Escherichia coli