CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F′ INCORPORATING THEM

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Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F′ incorporating them. J. Bacteriol. 87:554–561. 1964.—The gene responsible for thymine requirement or independence in Escherichia coli K-12 is located on the chromosome, near Arg2 (arginine) and between Sm (streptomycin) and Ade (adenine). A new F′ (called F15) carrying the Thy (thymine) gene as well as the Arg2 gene was discovered. The Thy+ (thymine independent) gene on F15 relieves the defect of all Thy− (thymine requiring) mutants of E. coli examined, such as E. coli 15 T−, B3, and Thy− mutants obtained by aminopterine treatment. It can also be transferred to Thy− mutants of Salmonella typhimurium and Serratia marcescens, converting them to Thy+.

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