cis Elements Necessary for Developmental Expression of a Myxococcus xanthus Gene That Depends on C Signaling

AUTOR(ES)

Viswanathan, Poorna

FONTE

American Society for Microbiology

RESUMO

Cell contact-mediated C signaling coordinates morphogenesis and gene expression during development of Myxococcus xanthus. One promoter that depends on C signaling for transcription lies upstream of Ω4403, the site of a Tn5 lac insertion in the genome. The Ω4403 promoter has a C-box sequence centered at −49 bp that matches the consensus 5′-CAYYCCY-3′, which is found in several C-signal-dependent promoters. Mutational analysis of the Ω4403 promoter region was performed to test the importance of the C box and to identify other cis-acting elements. A 6-bp change in the −10 region eliminated promoter activity, but a 6-bp change in the −35 region decreased activity only about twofold. Certain single-base-pair changes in the C box centered at −49 bp abolished promoter activity, establishing the importance of this sequence element. Single-base-pair changes in a C-box-like sequence centered at −77 bp also abolished promoter activity, but the pattern of mutational effects was different from that for the C box centered at −49 bp. Additional single-base-pair changes indicated that all 10 bp from −79 to −70 bp are important for Ω4403 promoter activity. Mutations at −59, −61, −62, and −63 bp also abolished promoter activity, defining a 5-bp element from −63 to −59 bp. This 5-bp element is separated from the 10-bp element (i.e., −79 to −70 bp) by 6 bp that can be changed without loss of promoter activity. Likewise, the 5 bp between the 5-bp element and the C box can be changed without loss of activity, but deletion of these 5 bp abolished activity, indicating that spacing is important. Sequences similar to the 5- and 10-bp elements, as well as the C box, are present in other C-signal-dependent promoters, suggesting some similarity in the regulatory mechanisms, but there are also indications that these cis elements do not function identically in the different promoters.

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