Classification of fungal chitin synthases.
AUTOR(ES)
Bowen, A R
RESUMO
Comparison of the chitin synthase genes of Saccharomyces cerevisiae CHS1 and CHS2 with the Candida albicans CHS1 gene (UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, EC 2.4.1.16) revealed two small regions of complete amino acid sequence conservation that were used to design PCR primers. Fragments homologous to chitin synthase (approximately 600 base pairs) were amplified from the genomic DNA of 14 fungal species. These fragments were sequenced, and their deduced amino acid sequences were aligned. With the exception of S. cerevisiae CHS1, the sequences fell into three distinct classes, which could represent separate functional groups. Within each class phylogenetic analysis was performed. Although not the major purpose of the investigation, this analysis tends to confirm some relationships consistent with current taxonomic groupings.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48270Documentos Relacionados
- Stigmatella aurantiaca fruiting body formation is dependent on the fbfA gene encoding a polypeptide homologous to chitin synthases.
- Properties of subcloned subunits of bacterial acetohydroxy acid synthases.
- Modification of human U4 RNA requires U6 RNA and multiple pseudouridine synthases.
- Effect of calcofluor white on chitin synthases from Saccharomyces cerevisiae.
- New members of the barley Kas gene family encoding beta-ketoacyl-acyl carrier protein synthases.