Clinical evaluation of commercial conjugates for direct immunofluorescence of herpes simplex virus.
AUTOR(ES)
Gittzus, J G
RESUMO
Commercially prepared antibody conjugates that are now available allow extensive application of direct immunofluorescence for diagnosing herpetic infections. Five of these conjugates--Microbiological Associates bivalent herpes antiserum (MBA-bivalent), MBA anti-herpes types 1 (MBA-1) and 2 (MBA-2), and Flow Laboratories anti-herpes types 1 (Flow-1) and 2 (Flow-2)--were compared for antibody titer, diagnostic accuracy, and nonspecific background staining on known infected tissue. Four of these were tested simultaneously on 75 clinical specimens. No attempt was made to differentiate between herpes simplex virus type 1 or type 2 infections. All antisera were comparable in titer and exhibited fluorescence with both herpes simplex virus types 1 and 2 antigens. The most significant difference was excessive nonspecific staining consistently seen with the MBA-2 conjugate. Overall, Flow-2 and MBA-bivalent antisera were preferable.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=274827Documentos Relacionados
- Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.
- Evaluation of three immunofluorescence assays for culture confirmation and typing of herpes simplex virus.
- Evaluation of a commercial enzyme-linked immunosorbent assay for the detection of herpes simplex virus.
- Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence.
- Comparison of immunofluorescence with commercial monoclonal antibodies to biochemical and biological techniques for typing clinical herpes simplex virus isolates.