Clonal Analysis of the Human B-Cell Repertoire Using a Heteromobility Assay
AUTOR(ES)
Tewari, Deepanker
FONTE
American Society for Microbiology
RESUMO
A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95903Documentos Relacionados
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