Cloning and characterization of araA, araB, and araD, the structural genes for L-arabinose utilization in Bacillus subtilis.
AUTOR(ES)
Sá-Nogueira, I
RESUMO
Two recombinant plasmids, pSNL1 and pSNL2, carrying structural genes for L-arabinose utilization were isolated from a Bacillus subtilis gene library. Both plasmids complemented araD mutations in a Rec- B. subtilis strain and in Escherichia coli. Moreover, pSNL1 also complemented araB mutations in both species and efficiently transformed araA Rec+ B. subtilis strains to Ara+. Detailed physical mapping of both plasmids in addition to transformation experiments involving defined restriction fragments from the pSNL1 insert unambiguously determined the gene order to be araD, araB, and araA, an order different from that found in E. coli.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=210170Documentos Relacionados
- Isolation of constitutive mutants for L-arabinose utilization in Bacillus subtilis.
- Cloning, functional analysis, and transcriptional regulation of the Bacillus subtilis araE gene involved in L-arabinose utilization.
- Polarity in Gene araB of the l-Arabinose Operon in Escherichia coli B/r
- l-Arabinose Isomerase Formation in a Conditional Mutant of Gene araA of Escherichia coli B/r
- Isolation of Constitutive Mutants for l-Arabinose Utilization in Bacillus subtilis
