Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.

AUTOR(ES)

Bower, S

RESUMO

The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.

Documentos Relacionados

• Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
• Conservation of the Biotin Regulon and the BirA Regulatory Signal in Eubacteria and Archaea
• Cloning and characterization of the repressor gene of the Staphylococcus aureus lactose operon.
• Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
• Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.