Cloning and constitutive expression of the N-acetylneuraminate lyase gene of Escherichia coli.
AUTOR(ES)
Aisaka, K
RESUMO
The N-acetylneuraminate (NANA) lyase (EC 4.1.3.3) gene from Escherichia coli was self-cloned in E. coli. Transformants were selected by complementation of a NANA lyase-deficient E. coli strain. One clone was found to produce NANA lyase, and it contained a recombinant plasmid, pNAL1, with a 9.0-kilobase HindIII insert. The cloning of the NANA lyase gene resulted in the change from inducible to constitutive production of the enzyme. The level of expression of the NANA lyase gene in E. coli(pNAL1) clones was two- to three-fold higher than that in the fully induced wild-type strains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=238919Documentos Relacionados
- Genetic and molecular analyses of Escherichia coli N-acetylneuraminate lyase gene.
- Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.
- Distribution of Neuraminidase and N-Acetylneuraminate Lyase Activities Among Corynebacteria, Mycobacteria, and Nocardias
- Mimicking natural evolution in vitro: An N-acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity
- Mucin degradation in the human colon: production of sialidase, sialate O-acetylesterase, N-acetylneuraminate lyase, arylesterase, and glycosulfatase activities by strains of fecal bacteria.