Cloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.).

AUTOR(ES)

Link, G

RESUMO

Restriction fragments of chloroplast (cp)DNA from mustard leaves were cloned in E. coli using pBR 322 as the vehicle. Cloned fragments containing the structural sequences for two polypeptides of 56,000 and 35,000 daltons were selected by a mRNA hybridization-translation procedure. The cloned mustard genes are structurally related to chloroplast genes from maize. They hybridize to the maize mRNAs for the large subunit of ribulosebisphosphate carboxylase and for the 34,500 dalton precursor to a membrane protein. The coding sequence on mustard cpDNA for the 56,000 dalton polypeptide is colinear with a 1,500 base pair transcript, and the sequence for the 35,000 dalton polypeptide is colinear with a 1,220 base pair transcript.

Documentos Relacionados

• Phosphorylation and dephosphorylation affect functional characteristics of chloroplast and etioplast transcription systems from mustard (Sinapis alba L.).
• Structure of the chloroplast gene for the precursor of the Mr 32,000 photosystem II protein from mustard (Sinapis alba L.).
• RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.).
• Ultraviolet Light Inhibition of Phytochrome-Induced Flavonoid Biosynthesis and DNA Photolyase Formation in Mustard Cotyledons (Sinapis alba L.).
• Nucleotide sequence of the chloroplast psbI and trnS-GCU genes from mustard (Sinapis alba).