Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
AUTOR(ES)
Boss, J M
RESUMO
The DC antigen is one of the class II major histocompatibility antigens involved in the regulation of the immune response. This molecule is a heterodimer composed of an alpha and a beta chain. Southern blot analysis of several homozygous cell lines shows that there are two DC beta genes. The DC-3 beta gene, corresponding to a polymorphic restriction fragment, was cloned and sequenced and found to exist in five exons spanning 8 kilobase pairs of DNA. These exons correspond to the functional domains of the DC beta protein. Comparison of the beta 1 domains of known DC beta chains shows that the polymorphism is clustered in four regions. A similar comparison of the mouse A beta sequences shows only two prominent diversity regions. The DC beta chain sequences are eight amino acids shorter than the A beta chain sequences due to the elimination of a small exon by an aberrant splice acceptor.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391665Documentos Relacionados
- Gene organization of DC and DX subregions of the human major histocompatibility complex.
- Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.
- Sequence analysis of the human major histocompatibility gene SX alpha.
- The Genomic Sequence and Comparative Analysis of the Rat Major Histocompatibility Complex
- DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
