Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B.

AUTOR(ES)
RESUMO

A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.

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