Cloning of a Vibrio alginolyticus rpoN gene that is required for polar flagellar formation.

AUTOR(ES)

Kawagishi, I

RESUMO

A fragment of DNA was cloned which complemented a polar flagellum-defective (pof) mutation of Vibrio alginolyticus. The fragment contained two complete and two partial open reading frames (ORFs) (ORF2 and -3 and ORF1 and -4, respectively). The presumed product of ORF2 has an amino acid sequence with a high degree of similarity to that of RpoN, which is an alternative sigma factor (sigma54) for other microorganisms. The other ORFs are also homologous to the genes adjacent to other rpoN genes. Deletion analysis suggests that ORF2 complements the pof mutation. These results demonstrate that RpoN is involved in the expression of polar flagellar genes.

Documentos Relacionados

• Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.
• The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene.
• RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.
• Physical map location of the rpoN gene of Escherichia coli.
• Physical Map Location of the rpoN Gene of Escherichia coli