Cloning of an Arabidopsis Patatin-Like Gene, STURDY, by Activation T-DNA Tagging

AUTOR(ES)

Huang, Shihshieh

FONTE

American Society of Plant Physiologists

RESUMO

Activation T-DNA tagging can generate dominant gain-of-function mutants by overexpression of a particular endogenous gene. We identified an activation-tagged mutant, sturdy, exhibiting a stiff inflorescence stem, thicker leaves, shorter siliques, larger seeds, round-shaped flowers, and delayed growth. It is most important that unlike its wild-type counterpart, this mutant is less prone to lodging. Cloning of STURDY revealed that in sturdy, there is an open reading frame containing a single intron encoding a patatin-like homolog. The T-DNA is inserted into the 3′ region of the second exon. The mutant phenotype was shown to be the result of overexpression of STURDY by mRNA analysis and transgenic studies. Preliminary histological studies have revealed an increase in cell number in the inflorescence stem of mutant plants; however, additional studies are needed to better understand the overexpression phenotype.

Documentos Relacionados

• Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana.
• Trichome Development in Arabidopsis thaliana. I. T-DNA Tagging of the GLABROUS1 Gene.
• T-DNA Insertional Mutagenesis for Activation Tagging in Rice1
• T-DNA as an insertional mutagen in Arabidopsis.
• Expression of a Patatin-like Protein in the Anthers of Potato and Sweet Pepper Flowers.