Cloning of cDNA encoding a 100 kDa nucleolar protein (nucleoline) of Chinese hamster ovary cells.
AUTOR(ES)
Lapeyre, B
RESUMO
Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing. We reported the isolation of 5 cDNA clones by probing a cDNA library, constructed in the expression vector lambda gt11, with a polyclonal serum raised against nucleoline. A new immunoassay, using hybrid proteins (beta gal-cDNA encoded protein) was developed to establish that the isolated cDNAs encoded parts of nucleoline. A further confirmation resulted from the sequence comparison between the cDNA encoded peptide and a 42 aa peptide isolated from rat nucleoline (1). The 5 cDNAs overlapped extensively and covered more than 90% of a full length cDNA. By probing a Northern blot with the 100 kDa cDNA, a 2650 nucleotide polyA+ RNA was detected that contained just enough information to code for nucleoline.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321913Documentos Relacionados
- Mimosine, a novel inhibitor of DNA replication, binds to a 50 kDa protein in Chinese hamster cells.
- A Chinese hamster ovary cyclophilin cDNA sequence.
- Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.
- Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.
- Cloning and characterization of small circular DNA from Chinese hamster ovary cells.