Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor.
AUTOR(ES)
Jenny, A
RESUMO
3'-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor or as well as for the tail elongation reaction. We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. The cDNAs code for an open reading frame of 1444 amino acids. The translated protein has a calculated molecular weight of 161 kDa and a predicted pl of 6.2. Polyclonal antibodies raised against a bacterially expressed fragment of the cDNA recognise 160 kDa subunit of purified calf thymus CPSF. The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=307085Documentos Relacionados
- Isolation of cDNAs encoding the Drosophila GAGA transcription factor.
- cDNAs encoding the large subunit of human replication factor C.
- Cloning and characterization of two cDNAs coding for human von Willebrand factor.
- Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit.
- Cloning and sequencing of cDNAs encoding the two subunits of Crotoxin.