Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.
AUTOR(ES)
Williams, D M
RESUMO
Plasmid pPL603 (3.1 megadaltons) specifies neomycin resistance in Bacillus subtilis and contains a structural gene for chloramphenicol acetyltransferase. Cells harboring the plasmid cannot grow on solid media containing 10 microgram of chloramphenicol per ml. Cloning EcoRI (or EcoRI)-generated fragments of deoxyribonucleic acid from several sources into the single EcoRI site in plasmid pPL603, with subsequent selection of transformants of media containing 10 micrograms of chloramphenicol per ml, permits the identification of restriction fragments that promote expression of the chloramphenicol acetyltransferase gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216974Documentos Relacionados
- Restriction fragments that exert promoter activity during postexponential growth of Bacillus subtilis.
- Cloning and expression of the Bacillus anthracis protective antigen gene in Bacillus subtilis.
- Cloning of the Bacillus subtilis sulfanilamide resistance gene in Bacillus subtilis.
- Cloning the gyrA gene of Bacillus subtilis.
- Identification of specific restriction fragments associated with a membrane subparticle from Bacillus subtilis.