Cloning, sequencing, expression, and complementation analysis of the Escherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame encoding a protein with homology to GutQ.
AUTOR(ES)
Cieslewicz, M J
RESUMO
The kps locus for polysialic acid capsule expression in Escherichia coli K1 is composed of a central group of biosynthetic neu genes, designated region 2, flanked on either side by region 1 or region 3 kps genes with poorly defined functions. Chromosomal mutagenesis with MudJ and subsequent complementation analysis, maxicell and in vitro protein expression studies, and nucleotide sequencing identified the region 1 gene, kpsE, which encodes a 39-kDa polypeptide. Polarity of the kpsE::lacZ mutation suggests an operonic structure for region 1. KpsE is homologous to putative polysaccharide-translocation components previously identified in Haemophilus influenzae type b and Neisseria meningitidis group B. An open reading frame upstream of kpsE encodes a 35-kDa polypeptide with homology to GutQ, a putative ATP-binding protein of unknown function encoded by gutQ of the glucitol utilization operon. Whether expression of the gutQ homolog as the potential first gene of region 1 is required for polysialic acid synthesis or localization is presently unknown.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206983Documentos Relacionados
- Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.
- Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region.
- Cloning, sequencing, expression, and protective capacity of the oma87 gene encoding the Pasteurella multocida 87-kilodalton outer membrane antigen.
- Cloning, sequencing, and expression of the Pseudomonas testosteroni gene encoding 3-oxosteroid delta 1-dehydrogenase.
- Molecular Cloning, Sequencing, Expression, and Characterization of an Immunogenic 43-Kilodalton Lipoprotein of Bartonella bacilliformis That Has Homology to NlpD/LppB