Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor increases human immunodeficiency virus-1 DNA vaccine-induced polyfunctional CD4+ T-cell responses
AUTOR(ES)
Santana, Vinicius Canato, Almeida, Rafael Ribeiro, Ribeiro, Susan Pereira, Ferreira, Luís Carlos de Souza, Kalil, Jorge, Rosa, Daniela Santoro, Cunha Neto, Edecio
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
24/11/2015
RESUMO
T-cell based vaccines against human immunodeficiency virus (HIV) generate specific responses that may limit both transmission and disease progression by controlling viral load. Broad, polyfunctional, and cytotoxic CD4+T-cell responses have been associated with control of simian immunodeficiency virus/HIV-1 replication, supporting the inclusion of CD4+ T-cell epitopes in vaccine formulations. Plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) co-administration has been shown to induce potent CD4+ T-cell responses and to promote accelerated priming and increased migration of antigen-specific CD4+ T-cells. However, no study has shown whether co-immunisation with pGM-CSF enhances the number of vaccine-induced polyfunctional CD4+ T-cells. Our group has previously developed a DNA vaccine encoding conserved, multiple human leukocyte antigen (HLA)-DR binding HIV-1 subtype B peptides, which elicited broad, polyfunctional and long-lived CD4+ T-cell responses. Here, we show that pGM-CSF co-immunisation improved both magnitude and quality of vaccine-induced T-cell responses, particularly by increasing proliferating CD4+ T-cells that produce simultaneously interferon-γ, tumour necrosis factor-α and interleukin-2. Thus, we believe that the use of pGM-CSF may be helpful for vaccine strategies focused on the activation of anti-HIV CD4+ T-cell immunity.
Documentos Relacionados
- Specific human granulocyte-macrophage colony-stimulating factor antagonists.
- Granulocyte-Macrophage Colony-Stimulating Factor in Staphylococcus aureus-Induced Arthritis
- Regulation of human monocyte adherence by granulocyte-macrophage colony-stimulating factor.
- Effect of granulocyte-macrophage colony-stimulating factor in experimental visceral leishmaniasis.
- T-cell nuclei contain a protein that binds upstream of the murine granulocyte-macrophage colony-stimulating factor gene.