Comparison of a single-antigen microimmunofluorescence assay and inclusion fluorescent-antibody assay for detecting chlamydial antibodies and correlation of the results with neutralizing ability.
AUTOR(ES)
Peterson, E M
RESUMO
An inclusion fluorescent-antibody assay (IFA) with McCoy cells infected with Chlamydia trachomatis serovar L2 was compared with a single-antigen (L2) microimmunofluorescence (MIF) assay for the detection of antichalmydial antibodies. A total of 562 serum specimens were tested by both assays, and sera representing a range of titers were tested for their ability to neutralize the infectivity of C. trachomatis. Overall, there was poor correlation between the two assays (r2 = 0.62). With most sera the inclusion IFA was more sensitive. There was better correlation between IFA titer and ability to neutralize the five serovars tested (L2, L3, C, E, and F) than between the MIF assay and neutralization. In summary, the IFA appeared to be more sensitive than the MIF assay for detecting antibodies to C. trachomatis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267310Documentos Relacionados
- Single-antigen immunofluorescence test for chlamydial antibodies.
- Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii.
- Inclusion Fluorescent-Antibody Test as a Screening Assay for Detection of Antibodies to Chlamydia pneumoniae
- Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.
- Soluble Antigen Fluorescent-Antibody Technique