COMPARISON OF CYTOCHROMES IN MYCOBACTERIA GROWN IN VITRO AND IN VIVO
AUTOR(ES)
Kusaka, Takashi
RESUMO
Kusaka, Takashi (National Institute for Leprosy Research, Tokyo, Japan), Ryo Sato, and Ko Shoji. Comparison of cytochromes in mycobacteria grown in vitro and in vivo. J. Bacteriol. 87:1383–1388. 1964.—Spectrophotometric investigations were made on cell suspensions or particulate fractions of four species of mycobacteria which had been cultivated in vitro. The results obtained indicated the presence in Mycobacterium smegmatis of cytochromes of the a, b1, and c types, as well as a CO-binding pigment similar to cytochrome o; in BCG and M. avium, cytochromes of the a, b, and c types were present; and, in M. paratuberculosis, cytochromes of the a, b1, and c types were present. Although no clear interrelations were evident between the cytochrome patterns of these organisms and the ease with which they can be cultivated in vitro, it was found that the total reduced nicotinamide adenine dinucleotide oxidase activity of the particulate fraction was remarkably low in M. paratuberculosis, which can be grown in vitro only with great difficulty. The cells of BCG grown in vivo and those of M. lepraemurium isolated from leprous nodules of mice were found to be completely deficient in cytochrome pigments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=277214Documentos Relacionados
- Heat Inactivation of In Vivo- and In Vitro-Grown Mycobacteria in Meat Products
- Comparison of cytochromes from anaerobically and aerobically grown cells of Pseudomonas perfectomarinus.
- Comparison of regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in hepatoma cells grown in vivo and in vitro.
- BIOCHEMICAL DIFFERENTIATION OF MYCOBACTERIUM TUBERCULOSIS GROWN IN VIVO AND IN VITRO12
- CHARACTERISTICS OF A STRAIN OF STAPHYLOCOCCUS AUREUS GROWN IN VIVO AND IN VITRO