Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.
AUTOR(ES)
Linsley, P S
RESUMO
The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=369830Documentos Relacionados
- Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.
- Differentiation of schistosomes by species, strain, and sex by using cloned DNA markers.
- Genetic mapping of mutations using phenotypic pools and mapped RAPD markers.
- Comparison of Two Alternative Dominant Selectable Markers for Wine Yeast Transformation
- Recombinant retroviruses encoding cell surface antigens as selectable markers.