Comparison of the Sensitivities of Two Escherichia coli Genes to In Vivo Variation of Lrp Concentration
AUTOR(ES)
Chen, ChangFeng
FONTE
American Society for Microbiology
RESUMO
Transcription of the Escherichia coli genes serA and gltBDF depends on the leucine-responsive regulatory protein, Lrp, and is very much decreased in an lrp mutant. By the use of an Lrp-deficient host and the lrp gene cloned under a plasmid-borne arabinose pBAD promoter, we varied the amount of Lrp present in the cell and showed that both genes were transcribed in proportion to the amount of Lrp synthesized. The affinity of serA for Lrp was four to five times greater than the affinity of gltD. Overproduction of Lrp was lethal to the cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106935Documentos Relacionados
- In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli.
- Differential binding of Lrp to two sets of pap DNA binding sites mediated by Pap I regulates Pap phase variation in Escherichia coli.
- Lrp stimulates phase variation of type 1 fimbriation in Escherichia coli K-12.
- ilvIH Operon Expression in Escherichia coli Requires Lrp Binding to Two Distinct Regions of DNA
- Regulation of the Escherichia coli lrp gene.
