Comparison of Tissue Preparation Methods for Assay of Nicotinamide Coenzymes
AUTOR(ES)
Zhao, Zhifan
RESUMO
To prepare tissues for analysis of NAD+, NADH, NADP+, and NADPH, common practice is to freeze samples in liquid nitrogen, often followed by freeze-drying, before extraction in HCl or NaOH. With cucumber (Cucumis sativus L.) cotyledons, prefreezing in liquid nitrogen or slower freezing to −20°C yielded substantially lower values for NADH and NADPH than obtained from samples homogenized immediately in acid or base. Freeze-drying after freezing in liquid nitrogen generally caused even lower values of those coenzymes. We suggest that direct extraction is more likely to yield accurate results with cotyledons and other plant parts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1056713Documentos Relacionados
- Comparison of microbiologic assay methods for hemodialysis fluids.
- Estimation of Nicotinamide Nucleotide Coenzymes in Etiolated Maize Seedlings 123
- Comparison of several fixation methods for cytomegalovirus antigenemia assay.
- Comparison of five methods for the assay of serum gentamicin.
- Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay.