Complementation of Amoebal-Plasmodial Transition Mutants in PHYSARUM POLYCEPHALUM
AUTOR(ES)
Anderson, Roger W.
RESUMO
Amoebae of the Myxomycete Physarum polycephalum differentiate to yield plasmodia in two ways: in crossing, haploid amoebae of appropriate genotypes fuse to form diploid plasmodia; in selfing, plasmodia form without amoebal fusion or increase in ploidy. Amoebae carrying the mating-type allele matAh (formerly mth) self efficiently, but occasionally give rise to mutants that self at very low frequencies. Such "amoebal-plasmodial transition" mutants were mixed in pairs to test their ability to complement one another in the formation of plasmodia by crossing. The pattern of crossing permitted 33 mutants to be assigned to four complementation groups (aptA-, npfA-, npfB- and npfC-). Similar tests had previously proved only partially successful, as crossing had occurred only rarely in mixtures of compatible strains. The efficiency of complementation was greatly increased in the current work by mixing strains that carried different alleles of a newly-discovered mating-compatibility locus, matB; this locus had no effect on the specificity of complementation. A possible interpretation of the complementation behavior of the mutants is suggested.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1216840Documentos Relacionados
- Differential gene expression during the amoebal-plasmodial transition in Physarum.
- Viability of Physarum polycephalum spores and ploidy of plasmodial nuclei.
- A plasmodial specific mRNA (plasmin C) from Physarum polycephalum encodes a small hydrophobic cysteine-rich protein.
- Selective initiation of replication at origin sequences of the rDNA molecule of Physarum polycephalum using synchronous plasmodial extracts.
- Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication.